Perform routine cell-based ELISA assays to evaluate in vitro relative protein expression (IVRPE) across development, characterization, release, and stability samples.
Culture and maintain mammalian cell lines using aseptic technique, ensuring optimal cell health, confluency, and viability.
Prepare assay plates, execute transfections, and manage multi-day workflows with precision and consistency.
Conduct serial dilutions, reagent preparation, sample handling, and plate-based assay execution in alignment with established protocols.
Monitor assay performance and respond to routine experimental issues with limited oversight, applying strong troubleshooting skills.
Operate laboratory instrumentation including plate readers, plate washers, incubators, biosafety cabinets, and associated equipment.
Acquire, review, and analyze experimental data using established tools, including curve fitting methodologies.
Maintain accurate, organized, and inspection-ready records within laboratory systems and notebooks.
Provide day-to-day technical guidance to Research Associates on assay execution, laboratory best practices, and troubleshooting fundamentals.
Troubleshoot experimental challenges, support investigations, and proactively identify opportunities to enhance assay robustness, efficiency, and scalability.
Lead or contribute to optimization of assay protocols and workflows to improve data quality, throughput, and readiness for automation.
Support method transfers and the implementation of automated workflows, leveraging digital tools and opportunities to engage with emerging generative AI technologies to enhance analytical insights.
Perform additional responsibilities as needed to support evolving Technical Development priorities.